Novel self-assembled tellurium nanoparticles and their effects in inhibition of myoblastoma cell line

Pandian  Chithamparam; Johnson Retnaraj Samuel Selvan  Christyraj; Senthil Muthu Kumar  Thiagamani; Malinee  Sriariyanun; Azhaguchamy  Muthukumaran

doi:10.6180/jase.202511_28(11).0017

Novel self-assembled tellurium nanoparticles and their effects in inhibition of myoblastoma cell line

Material Engineering Mechanical Engineering

Pandian Chithamparam¹, Johnson Retnaraj Samuel Selvan Christyraj², Senthil Muthu Kumar Thiagamani^3,4,5This email address is being protected from spambots. You need JavaScript enabled to view it., Malinee Sriariyanun⁶, and Azhaguchamy Muthukumaran¹This email address is being protected from spambots. You need JavaScript enabled to view it.

¹Department of Biotechnology, Kalasalingam Academy of Research and Education, Krishnankoil, Tamil Nadu, India

²Regeneration and Stem Cell Biology Lab, Centre for Molecular and Nanomedical Sciences, International Research Centre, Sathyabama Institute of Science and Technology, Chennai- 600119, Tamil Nadu, India.

³Department of Mechanical Engineering, Kalasalingam Academy of Research and Education, Anand Nagar, Krishnankoil 626126, Tamil Nadu, India.

⁴Department of Mechanical Engineering, INTI International University, Persiaran Perdana BBN, Putra Nilai, 71800 Nilai, Negeri Sembilan, Malaysia.

⁵Centre for Advanced Composite Materials (CACM), UniversitiTeknologi Malaysia, 81310 Skudai, Johor Bahru, Johor, Malaysia.

⁶Biorefinery and Process Automation Engineering Center, Department of Chemical and Process Engineering, The Sirindhorn International Thai-German Graduate School of Engineering, King Mongkut’s University of Technology North Bangkok, Bangkok, Thailand

Received: October 10, 2024
Accepted: January 17, 2025
Publication Date: March 16, 2025

Copyright The Author(s). This is an open access article distributed under the terms of the Creative Commons Attribution License (CC BY 4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are cited.

Download Citation: ||https://doi.org/10.6180/jase.202511_28(11).0017

Harnessing the distinct features of nanoparticles and nanomaterials for biomedical use has seen remarkable progress in recent years. Tellurium nanoparticles (TeNPs) have attracted attention due to their exceptional properties and potential uses in photoconductivity, nonlinear optics, piezoelectricity, and thermal conductivity applications. This study synthesized TeNPs through a novel heat reduction method using ascorbic acid as a reducing agent, followed by various characterization studies. Functional studies, such as the antibacterial activity, antioxidant activity and cytotoxicity assays, AO/EB staining, and live cell imaging were performed using TeNPs. The various concentrations of TeNPs (5−20 µg/ml) result in inhibition zones against S.pyogens in the 5-13 mm range and against E.coli in the 6-12 mm range. The produced TeNPs minimum inhibitory concentration (MIC) demonstrated that these nanoparticles had antibacterial properties against the tested gram-positive and gram-negative bacterial strains. Moreover, TeNPs exhibit strong antioxidant properties and demonstrate high toxicity in C2C12 myoblastoma cells. Notably, at a concentration of 5 µg/ml, TeNPs exhibit potential toxicity. Live cell imaging and cell proliferation assay with high and lower concentrations of TeNPs retards cell growth and proliferation of C2C12 myoblastoma cells compared to control. Based on the results, due to the increased toxicity nature of TeNPs, lower concentrations below 5 µg might be used in scaffolds to enhance cell proliferation for regenerative medicine and tissue engineering applications.

Keywords: Health, Apoptosis, antimicrobial activity, antioxidant activity, tellurium nanoparticles, self-assembly

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