Journal of Applied Science and Engineering

Published by Tamkang University Press


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Malinee Sriariyanun1This email address is being protected from spambots. You need JavaScript enabled to view it., Marttin Paulraj Gundupalli1, Vanarat Phakeenuya2, Theerawut Phusamtisampan2, Yu-Shen Cheng3, Ponnusami Venkatachalam4

1Biorefinery and Process Automation Engineering Center, Department of Chemical and Process Engineering, The Sirindhorn International Thai-German Graduate School of Engineering, King Mongkut’s University of Technology North Bangkok, Bangkok, Thailand

2Department of Biotechnology, Faculty of Applied Science, King Mongkut’s University of Technology North Bangkok, Bangkok, Thailand

3Department of Chemical and Materials Engineering, National Yunlin University of Science and Technology, Douliu, Yunlin 64002, Taiwan

4Bioprocess Intensification Laboratory, Center for Bioenergy, School of Chemical & Biotechnology, SASTRA Deemed University, Thirumalaisamudram, Thanjavur 613401, India


Received: November 13, 2022
Accepted: March 8, 2023
Publication Date: June 17, 2023

 Copyright The Author(s). This is an open access article distributed under the terms of the Creative Commons Attribution License (CC BY 4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are cited.

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Cellulosic ethanol has been gaining high attention due to its potential to reduce the greenhouse gas emission and cut down the world dependence on fossil fuels. Biorefinery approach for cellulosic ethanol has advantages due to its non-food competing status, natural abundance and benefit to decrease the combustion of agricultural wastes after harvesting seasons. Due to the recalcitrant structure of lignocellulose biomass, pretreatment and hydrolysis are critical to determine the economic viability of the process because they influence the conversion rate of fermentable sugars and, subsequently, final product i.e. ethanol. Therefore, the design for the process to compromise fermentation and upstream process is also essential. With all constraints exist when using harsh conditions during pretreatment, the recombinant engineered microorganisms have been developed and applied as biocatalysts during fermentation. To achieve the maximum production efficiency, different strategies of recombinant engineered microbes include expression optimization to modify the metabolic pathway, modification of secretion and transportation routes, improvement of stress tolerance, and utilization of both C5 and C6 sugars. This review provides the development and current status of cellulosic ethanol production via biorefining process by genetic engineered microbes with a focus on the technological aspects. The remaining challenges, perspective, and economical feasibility of the process are also discussed.

Keywords: Biorefinery, Enzymatic saccharification, Ethanol, Fermentation, Lignocellulosic biomass, Pretreatment, Recombinant engineering

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