Anand Elumalai1 G. Mohan Ganesh This email address is being protected from spambots. You need JavaScript enabled to view it.2 and Kalaichelvan Gurumurthy 3
1 School of Bio-Sciences and Technology, Vellore Institute of Technology, Vellore-632 014, TN, India 2 Department of Structural and Geotechnical Engineering, Vellore Institute of Technology, Vellore-632014, TN, India 3 VIT School of Agricultural Innovations and Advance Learning (VAIAL), Vellore Institute of Technology, Vellore-632014. TN, India.
In recent years, concrete has become an important versatile construction material. This paper evaluates the strength obtained by concrete with the influence of bacteria. The bacterial strains were isolated from calcareous sludge and urea ware house. The bacterial strains were identified through 16srRNA gene sequencing as Bacillus pumilus and Bacillus flexus. Using these strains, an experiment on cylinder and prisms cast was performed. Compressive strength, split tensile and flexural tests were conducted at the age of 7, 28 and 56 days with ultrasonic pulse velocity and rebound hammer. The results were compared with Bacillus cohnii MTCC 3616 obtained from microbial type culture collection and gene bank, Chandigarh, India. Based on the experimental results, the improvement in the mechanical strength is due to the deposition of calcite crystals on the bacterial cell surfaces within the pores which enhanced the strength of concrete and reduced porosity and permeability.
Keywords: Biomineralization, Bacterial Concrete, Ultra sonic pulse velocity, Scanning electron microscope (SEM) and X-ray diffraction (XRD)
REFERENCES
[1]Warscheid, T. and J. Braams. (2000) Bio deterioration of stone: a review. International Bio deterioration & Biodegradation, 46(4): p. 343-368.
[2]Le Métayer-Levrel, G., et al., (1999) Applications of bacterial carbonatogenesis to the protection and regeneration of limestones in buildings and historic patrimony. Sedimentary Geology, 126(1-4): p. 25-34.
[3]Samani, A.K. and M.M. Attard, (2014) Lateral strain model for concrete under compression. ACI Structural Journal, 111(2): p. 441-451.
[4]Malhotra V. M., (2000) Introduction: Sustainable Development and Concrete technology, ACI Concrete Journal, pp.1147-1165.
[5]Sumajouw D.M., Hardjito D Wallah S.E., Rangan B.V. (2006) Fly ash based Geopolymer Concrete: Study of Slender Reinforced Columns, Advances in Geopolymer science and technology, pp. 3124-3130.
[6]Warren, L.A and Hacck, E.A. (2001).Biochemical controls on metal behaviour in freshwater environment. Earth-science Reviews, 54: 261-320.
[7]Tebo, B.M., et al., (2005) Geomicrobiology of manganese (II) oxidation. Trends in Microbiology, 13(9): p. 421-428.
[8]Rivadeneyra, M.A., et al., (1994) Precipitation of calcium carbonate by Vibrio spp. from an inland saltern. FEMS Microbiology Ecology 13(3): p. 197-204.
[9]Nagarajan V., et al., (2012) A Study on the Strength of the Bacterial Concrete Embedded with Bacillus Megaterium. International Research Journal of Engineering and Technology (IRJET) Volume: 04 Issue: 12.
[11]Mcgenity, T.J and Sellwood, B.W. (1999).New approaches to studying the microbial precipitation of carbonate mineral. Sedimentary geology, 126: 5-8.
[12]Barton, L.L. and D.E. Northup, (2011) Microbes at Work in Nature: Bio mineralization and Microbial Weathering, in Microbial Ecology. John Wiley & Sons, Inc.: Hoboken, NJ, USA. . p. 299-326.
[13]Hammes, F. and W. Verstraete, (2002) Key roles of pH and calcium metabolism in microbial carbonate precipitation.
[14]Fortin, D., F.G. Ferris, and T.J. Beveridge (1997) Surface-mediated mineral development by bacteria. Reviews in Mineralogy, 35: p. 176-180.
[15]Frederik hammes (2003) Strain specific Ureolytic microbial calcium carbonate precipitation. Applied and environmental microbiology .vol 69.no 8.pp 4902- 4909.
[16]Altschul, S.F; Madden, T.L; Schaffer, A.A; Zhang, J., Miller; W; Lipman,D.J, (1997) Gapped BLAST and PSI- BLAST. A new generation of protein database search program. Nucleic Acids Research, 25:3389-3402.
[17]Ordinary Portland Cement, 43 Grade- Specification, IS 8112:2013,(2013) Bureau of Indian standard, New Delhi.
[18]Mohan Ganesh G; Santhi A.S; Anand Elumalai; Kalaichelvan Gurumurthy; (2015)“Experimental Investigation of Bacterial Concrete to Improve Compressive Strength: A Microbiology and Civil Engineering Approach . The Indian Concrete Journal published by ACC / Vol.89, No.-6/ June 2015/Pp-24 -30.
[19]IS 383:1970. Specification for coarse and fine aggregates from natural sources for concrete, New Delhi, India: Bureau of Indian standards.
[20]IS 10262:2009. Method for concrete mix proportion sources for concrete, New Delhi, India: Bureau of Indian standards.
[21]IS 516 :1959. Indian standard code of practice- methods of test for strength of concrete, New Delhi, India: Bureau of Indian standards.
[22]IS 13311: 1992. Methods of non-destructive texting of concrete- methods of test, part 2: rebound hammer [CED 2: cement and concrete].
[23]Jiri Brozovsky, Lenka Bodnarova and Jiri Brozovsky Jr, (2019) Rebound Hammer Tests of High-Strength Concrete: Effects of Internal Stress and the Shape of the Impact Area of the Test Specimens on the Measurement Results, Periodica Polytechnica Civil Engineering, 63(1), pp. 215–221
[24]IS 5816 (1999): Method of Test Splitting Tensile Strength of Concrete [CED 2: Cement and Concrete]
[25]Vamsi Pradeep; Kishore Kumar (2013) The behaviour of concrete interms of flexural, tensile and compressive strength properties by using copper slag as an admixture. International Journal of Engineering and Innovative Technology (IJEIT) Volume 3, Issue 4, ISSN: 2277-3754.
[26]IS 516:1959. Indian standard code of practice- methods of test for strength of concrete, New Delhi, India: Bureau of Indian standards.
[27]Bashir J, Kathwari I, Tiwary A, Singh K (2016). Bio Concrete-The Self-Healing Concrete. Indian Journal of Science and Technology. ; 9 (47).
[28]Ghosh S, Biswas M, Chattopadhyay BD, Mandal S (2009). Microbial activity on the microstructure of bacteria modified mortar. Cem Concr Compos; 31:93–8.
We use cookies on this website to personalize content to improve your user experience and analyze our traffic. By using this site you agree to its use of cookies.
