Improvements of Acinetobacter Radioresistens Lipase Adsorption on Celite 535 by Adding Salts

Chia-Hui  Hsu; Shau-Wei Tsai

doi:10.6180/jase.2001.4.2.08

Improvements of Acinetobacter Radioresistens Lipase Adsorption on Celite 535 by Adding Salts

Chemical Engineering

Chia-Hui Hsu¹ and Shau-Wei Tsai¹

¹Department of Chemical Engineering National Cheng Kung University Tainan, Taiwan 701, R.O.C.

Received: January 1, 2001
Accepted: June 21, 2001
Publication Date: January 1, 2001

Download Citation: ||https://doi.org/10.6180/jase.2001.4.2.08

ABSTRACT

The zeta potential of Celite 535 powder in deionied water can be effectively increased by the addition of salts, especially the divalent ion Ca⁺⁺ or Mg⁺⁺. However, this enhancement was not observed in a sodium phosphate buffer near neutral pH, which implies that Ca⁺⁺ or Mg⁺⁺ can not compete with Na⁺ having adsorbed on the support surface. Improvements of the protein and lipase adsorption on Celite 535 were found when salts were added to the crude enzyme solution. The highest specific activity of 167% of the support (c.a. 2.5-fold enhancement by comparing with that without adding salts) was found when the enzyme solution contains 100 mM NH₄Cl. An S-shape adsorption isotherm for the lipase activity was found, which was attributed to the larger pores of the support. However, no purification effect was observed when the protein and lipase was precipitated by adding acetone to the enzyme solution containing 100 mM NH₄Cl.

Keywords: Lipase Adsorption, Celite 535, Salt Effects, Precipitation

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