Chemical Engineering

Bima Putra Pratama1, Yudi Pranoto This email address is being protected from spambots. You need JavaScript enabled to view it.1, Supriyadi1, and Respati Tri Swasono2

1Department of Food and Agricultural Product Technology, Faculty of Agricultural Technology, Universitas Gadjah Mada, Jl. Flora, Bulaksumur, Yogyakarta 55281, Indonesia
2Department of Chemistry, Faculty of Mathematics and Natural Sciences, Universitas Gadjah Mada, Jl. Sekip Utara, Yogyakarta 55281, Indonesia

 

Received: November 16, 2021
Accepted: February 8, 2022
Publication Date: July 29, 2022

 Copyright The Author(s). This is an open access article distributed under the terms of the Creative Commons Attribution License (CC BY 4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are cited.


Download Citation: ||https://doi.org/10.6180/jase.202304_26(4).0015  


ABSTRACT

Salam leaf (Syzygium polyanthum) is an Indonesian traditional herbs with high bioactive compound potential. Unfortunately, as an alternative method that is friendly to heat-susceptible bioactive compound, the ultrasonication extraction method has not been widely used to extract Salam leaves. Meanwhile, the processing time and the ratio of the use of solvents are some of the determinants of the performance of this method. Hence, this research examined the ultrasonication method for extracting bioactive compounds from Salam leaf from the time and ratio of the solvent used. The results were showed that the best condition was from the solvent ratio to sample of 12:1 for 15 min extraction time. These conditions gave results in an 18.57% yield of salam leaf extract, 74.20 ± 0.19 mg Gallic acid equivalent/g salam leaf extract of total phenolic content, 22.00 ± 0.08 mg Quercetin equivalent/g salam leave extract of total flavonoid content, 78.79% of 2,2-diphenyl-1-picrylhydrazyl (DPPH) scavenging activity, and IC50 antioxidant activity of 20.25 μg/ml salam leave extract. The best extraction conditions are able to provide Salam leaf extract with very strong antioxidant potential.


Keywords: Salam leaf, ultrasonication method, bioactive compound, antioxidant activity


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