Tongyu Li1, Chunying Li2, Binxia Xue This email address is being protected from spambots. You need JavaScript enabled to view it.1, Li Wei  3, Tong Zhang1, and Qingqing Feng4

1School of Architecture, Harbin Institute of Technology / Key Laboratory on Urban-Rural Human Settlement Environment in Winter City of Ministry of Science and Technology Industry and Information, Harbin, China. 150006
2School of Energy and Civil Engineering, Harbin University of Commerce, Harbin, China. 150028
3State Key Laboratory of Urban Water Resource and Environment, Harbin, China .150090
4Northeast Forestry University, Department of Landscape Architecture, Harbin, China. 100083


Received: September 2, 2020
Accepted: November 17, 2020
Publication Date: June 1, 2021

 Copyright The Author(s). This is an open access article distributed under the terms of the Creative Commons Attribution License (CC BY 4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are cited.

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In Daqing, years of oil exploration have led to severe oil pollution, aggravating salinization particularly. In this study, the concept of terraced landscape design combined with T. ambiguum Bieb. and microbial inoculums is proposed as a restoration technology for saline and alkali land, with the aim of removing oily substances and recovering soil fertility. The technology of 454 high-throughput sequencing was deployed to analyze the microbial community of the inoculums and the salinity and alkalinity changes of terraced land. The removal rate using T. ambiguum Bieb. and microbial inoculums was 94.7 % after seventy days. The dominant phyla for the saline and alkali soil after the treatment were Firmicutes (61.74 % of total bacteria) and Proteobacteria (35.91 %), At a general level, the relative abundances were Lactobacillus (6.22 %), Lysinibacillus (13.61 %), Clostridium (12.98 %), Clostridium XI (18.39 %), Lachnospiraceae (16.74 %) and Enterobacteriaceae (15.30 %). Microbes were reinforced biologically in advantageous clusters in microbial inoculums. In a landscape design for terraces that used the principle of gravity sedimentation, the pH approached 7, alkalinity was reduced by 50 % on average, and salinity was decreased by 75 % at the upper level, indicating a considerable decrease in salinity and alkalinity. The landscape measure of a trapezoidal terrace using natural sedimentation combined with plant-microbial inoculums offers a treatment technology and pattern for the protection and development of saline and alkali land in oil exploration areas.

Keywords: Saline-alkali land; T. ambiguum Bieb. and microbial inoculums; Combined restoration; Terraced landscape; Daqing


  1. [1] W. Zunqin, Z. Shouquan, and Y. Renpei. Saline soil in China (in Chinese). 1993.
  2. [2] Pichu Rengasamy. Soil processes affecting crop production in salt-affected soils. Functional Plant Biology, 37(7):613–620, 2010.
  3. [3] Jiuchun Yang, Shuwen Zhang, L. I. Ying, B. U. Kun, Yubo Zhang, Liping Chang, and Yangzhen Zhang. Dynamics of saline-alkali land and its ecological regionalization in western songnen plain, China. Chinese Geographical Science, 20(2):159–166, 2010.
  4. [4] Z. Lanpo, S. Qinglin, and L. Chunlin. Research and problems of the improvement and utilization of sodic soil status in Songliao Plain. Journal of Jilin Agricultural University (in Chinese), 22:79–85, 2000.
  5. [5] Wei Zhao, Qin Zhou, Zongze Tian, Yutong Cui, Ying Liang, and Hongyan Wang. Apply biochar to ameliorate soda saline-alkali land, improve soil function and increase corn nutrient availability in the Songnen Plain. Science of the Total Environment, 722, 2020.
  6. [6] Z. Plaut, M. Edelstein, and M. Ben-Hur. Overcoming Salinity Barriers to Crop Production Using Traditional Methods, jul 2013.
  7. [7] L: Dongxing and G. Weiguang. Evaluation of site quality for saline-alkali soil in Daqing. Science of Soil and Water Conservation (in Chinese), 3, 2009.
  8. [8] Yang-Ren WANG, Shao-Zhong KANG, Fu-Sheng LI, Lu ZHANG, and Jian-Hua ZHANG. Saline Water Irrigation Scheduling Through a Crop-Water-Salinity Production Function and a Soil-Water-Salinity Dynamic Model. Pedosphere, 17(3):303–317, 2007.
  9. [9] P. Baoyuan, G. Weiguang, Z. Zifeng, and L. Chang. Classification and Evaluation of the Soda-saline Soil in Daqing. Journal Northeast Forestry University (in Chinese), 34(2):57, 2006.
  10. [10] K. F. Zhao, W. J. Zhang, H. Fan, J. Song, and X. Y. Jiang. Biological measures for utilization and development of salinized soil. Chinese Journal of Soil Science, 32(S1):115– 119, 2011.
  11. [11] C. Enfeng, W. Ruyong, and W. Chunyu. The Role of Organic Improvement Saline-Alkali Land. Chinese Journal of Soil Science, 18(5):193–196, 1984.
  12. [12] Luke Beesley, Eduardo Moreno-Jiménez, Jose L. Gomez-Eyles, Eva Harris, Brett Robinson, and Tom Sizmur. A review of biochars’ potential role in the remediation, revegetation and restoration of contaminated soils, 2011.
  13. [13] Sumia Khan, Muhammad Afzal, Samina Iqbal, and Qaiser M. Khan. Plant-bacteria partnerships for the remediation of hydrocarbon contaminated soils, 2013.
  14. [14] Mang Lu, Zhongzhi Zhang, Wei Qiao, Xiaofang Wei, Yueming Guan, Qingxia Ma, and Yingchun Guan. Remediation of petroleum-contaminated soil after composting by sequential treatment with Fenton-like oxidation and biodegradation. Bioresource Technology, 101(7):2106–2113, 2010.
  15. [15] Wanting Ling, Hongjiao Dang, and Juan Liu. In situ gradient distribution of polycyclic aromatic hydrocarbons (PAHs) in contaminated rhizosphere soil: A field study. Journal of Soils and Sediments, 13(4):677–685, 2013.
  16. [16] Zhong Min Jin, Wei Sha, Yan Fu Zhang, Jing Zhao, and Hongyang Ji. Isolation of Burkholderia cepacia JB12 from lead-and cadmium-contaminated soil and its potential in promoting phytoremediation with tall fescue and red clover. Canadian Journal of Microbiology, 59(7):449–455, 2013.
  17. [17] Bin xia Xue, Tong yu Li, Chun ying Li, Li Wei, and Xiao he Hou. Study on the sediment characteristics of the snow in a typical residential community and its integrated biological treatment pattern in Harbin. Environmental Science and Pollution Research, 24(32):25010– 25020, nov 2017.
  18. [18] Narayana Murthy Venkatesh and Nagarajan Vedaraman. Remediation of soil contaminated with copper using Rhamnolipids produced from Pseudomonas aeruginosa MTCC 2297 using waste frying rice bran oil. Annals of Microbiology, 62(1):85–91, mar 2012.
  19. [19] Bo Ding, Ya Ping Zhang, and Oliver A. Ryder. Extraction, PCR amplification, and sequencing of mitochondrial DNA from scent mark and feces in the giant panda. Zoo Biology, 17(6):499–504, 1998.
  20. [20] Susan M. Huse, Julie A. Huber, Hilary G. Morrison, Mitchell L. Sogin, and David Mark Welch. Accuracy and quality of massively parallel DNA pyrosequencing. Genome Biology, 8(7), jul 2007.
  21. [21] J. R. Cole, Q Wang, E Cardenas, J. Fish, B. Chai, R. J. Farris, A. S. Kulam-Syed-Mohideen, D. M. McGarrell, T. Marsh, G. M. Garrity, and J. M. Tiedje. The Ribosomal Database Project: Improved alignments and new tools for rRNA analysis. Nucleic Acids Research, 37(SUPPL. 1), 2009.